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WebIsolation and subfractionation on ficoll gradients of adult rat hepatocytes. Size, morphology, and biochemical characteristics of cell fractions. P Drochmans, P Drochmans Search for … WebApr 6, 2024 · Blood was diluted with PBS and PBMCs were obtained by density gradient centrifugation using Ficoll solution (density of 1.077, Biocoll Separating Solution, Biochrom AG, Berlin, Germany). ... Biochrom AG, Berlin, Germany). PBMCs (10 6 /mL) from healthy dogs were added to cells infected with rNDV in 24-well plates. PBMCs were collected … WebPeripheral blood mononuclear cells (PBMCs) were obtained from HD and MM patients by Ficoll gradient. PBMCs (1 × 10 6 cells/mL) were cultured in RPMI 1640 medium (Biochrom GmbH, Berlin, Germany) with 10% fetal bovine serum (FBS) (Biochrom GmbH, Holliston, MA, USA) and 20 ng/mL human IL-3 (Miltenyi Biotec, Bergisch Gladbach, Germany). bing rewards powershell script