Phis2 载体

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August undefined, 2024

Webb16 mars 2024 · 诱饵载体：pHis2-DNA（筛选标记Trp报告基因His）文库载体：pGADT7-cDNA（筛选标记Leu）实验步骤：一、转化诱饵载体（参考SK2400中质粒转化步骤） 1. YPDA平板培养划线，活化Y187酵母菌种；挑酵母菌落于液体YPDA培养基摇菌培养； 2. 制备感受态并将pHis2-DNA转化进入感受态细胞； 3. SD/-Trp平板检测转化结果。二、自 … Webb8 juni 2014 · 本研究根据植物胚柄特异表达基G564启动子关键序列(GAAAAGCGAA)合成次串联重复序列，并与报告质粒pHIS2．1连接构建诱饵载体pHIS2．1．G564．5A；以纯化的拟南芥rabidopsisthaliana)幼嫩种子mRNA模板，合成SMARTTM和CDS1I锚定末端dscDNA：并将dscDNA与线性化融合表达载体pGADT7一Rec2 ...

Y187-pHis2系统酵母单杂培养基套装说明书 - BIOON

Webbxho1和sac1的酶切位点是什么？它识别的特定基因是体内3广a泛存在的siRNA和miRNA前体。Dicer酶的PAZ结构域特异性地识别 0’端有 5个j碱基突出的 dsRNA,之w后由RNase III结构域剪切8生成siRNA或microRNA。 2011-10-27 8:58:59 Webb20 juni 2024 · 产品详细描述载体基本信息 Clontech pHis2 酵母单杂交载体 -- -- 多克隆位点，限制性内切酶 7207bp -- -- -- 卡纳霉素 -- 含HIS3报告基因 -- -- -- -- 载体质粒图谱和多克 … church of christ publishing house

PT3705-5 (PR32049) pHIS2

Webb1 juni 2024 · 载体描述. pYES2的是一个5.9 kb的载体，设计用来在酿酒酵母（Saccharomyces cerevisiae）中诱导表达重组蛋白。. 载体的特点在于基因插入载体的构建简单，以及能够使用原养型尿嘧啶进行转化株的筛选。. 该载体包含以下元素：. http://www.biofeng.com/zaiti/qita/pHISi.html http://www.honorgene.com/product_list/carrier_library/123112.html church of christ quincy il

WB800枯草芽孢杆菌表_价格-品牌-详情介绍_丁香通

WebbpHIS-2 Vector Database Welcome to Vector Database! Vector database is a digital collection of vector backbones assembled from publications and commercially available … Webb一、转化诱饵载体（参考Super转化试剂盒SK2402质粒转化步骤） 1.YPDA平板培养划线，活化Y187酵母菌种；挑酵母菌落于液体YPDA培养基摇菌培养； 2.制备感受态并 … dewalt miter saw cord replacementhttp://html.rhhz.net/SWJSTB/html/2024-12-110.htm church of christ programs on tv

"Webb质粒简介：. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker™ One-Hybrid Library Construction & Screening Kit (#K1617-1). It contains a HIS3 nutritional reporter gene,located downstream of a multiple ... " - Phis2 载体

Phis2 载体

p53-His2 plasmid,p53-His2,p53-His2 plasmid,p53-His2 sequence,p53-His2 …

http://www.bioon.com.cn/doc/showarticle.asp?newsid=96627 Webb更多详情咨询 www.bnbiotech.com, 021-61496710,15800960770 下面所有质粒和菌株，均为本公司实验室亲自验证过，可以提供技术支持，载体来源于promega,addgene,clontech,novagen,invitrogen可提供质粒图谱，可提供近万种质粒载体、菌株、基因和细胞株,并可提供实验技术外包服务,如基因克隆、质粒构建、蛋白原核、真 …

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WebbpHis2载体价格1000元，pHis2载体质粒图谱(Vector map),载体序列(Sequence)见下文，载体质粒抗性为Kanamycin，质粒大小为7207bp。pHis2是酵母单杂交载体的pHis2。 Webb26 maj 2024 · 载体描述. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker™ One- Hybrid Library Construction & Screening Kit (#K1617-1). It contains a HIS3 nutritional reporter gene, located downstream of a …

Webb1、提供详细的、完整的实验报告，清晰的实验图片. 2、在受体菌的阳性菌. 自激活和毒性验证. 1、pHis2-Bait或pAbAi-Bait质粒. 2、或者Bait序列，质粒由我们构建. 酵母质粒提取和测序. 酵母菌液或者具有菌斑的平板. 1、提供详细的、完整的实验报告. 2、筛选结果的测序 ... WebbpHIS2 Vector Information PT3705-5 Sold as part of Catalog No. 630304 Restriction Map and Multiple Cloning Site (MCS) of pHIS2 Vector. Unique restriction sites are in bold. *Note: The Sac II site is resistant to cleavage. Description pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding ...

WebbpHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was specifically designed for use with the … Webb1.构建载体目的基因融合连入pGADT7-Rec2(AD); 顺式作用元件连入pHis2或pHis2.1载体(顺式元件可以只连接一个拷贝，也可以连接3个串联的并测序确认无误)。 2.验证两载体分别于对应的空载体共转时，有无激活转录报告基因情况，大多酵母苗株有毒性。

Webb14 aug. 2015 · 伟通生物汇集了数以百计的克隆载体，包含常规原核表达载体、昆虫细胞表达载体、哺乳动物表达载体、酵母表达载体、慢病毒表达载体、腺病毒表达载体、腺相关病毒表达载体、逆转录病毒表达载体、信号通道报告系统等。

Webb产品名称：pHIS2酵母单杂交质粒. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker™ One-Hybrid Library Construction & … church of christ radio onlineWebb12 apr. 2024 · 方法：以启动子A构建到酵母单杂交启动子载体pHIS2，文库质粒为pGADT7-中山杉文库。材料： 1．启动子克隆：pHIS2-A。根据实验需求进行扩增并酶切构建载体，两端的EcoRI和 SacI酶切位点，序列如下： GAATTCAGTTGGAATCACATCTATCATCAGAATGAAAGACCTAAGAAAAAAATGGCA … church of christ questionsWebb12 apr. 2024 · 膜上载体蛋白属内在蛋白，它有选择地和膜一侧的分子或离子结合，形成载体-物质复合物，通过构象变化透过膜，把分子或离子释放到另一侧。载体蛋白三种类型 . 1、单向运输载体：催化分子或离子单方向跨膜运输，顺电化学势差进行。 church of christ questions and answersWebbClose You have no items in your shopping cart.. Home; Plasmid; p53- His2 Model: PVT11251 dewalt miter saw dust collection upgradehttp://www.bioon.com.cn/doc/showarticle.asp?newsid=101857 church of christ radio programshttp://www.biofeng.com/zaiti/jiaomu/pHis2.html dewalt miter saw fence replacementhttp://www.bioon.com.cn/reagent/show_product.asp?id=3900880 dewalt miter saw dw716 accessories